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Crystallization methods provide a means to expose protein/DNA samples to the supersaturated state usually by one or more of the following processes: 1. Facilitating gradual evaporation of solvent (e.g. organic solvents). 2. Achieving gradual desolvation of sample by polyalcohols or salts. This is also achieved by the presence of polyethylene glycol or organic solvents which lower the dielectric constant of solvent and thus also alter the bulk properties of water. 3. Variation of pH and temperature to reduce protein solubility. These processes must occur under conditions which do not signi cantly alter the structure or stability of the sample molecule. We will now describe three of the principal crystallization methods currently in use. Microdialysis is a low-volume form of dialysis ( 7) which is a process of diffusion across semipermeable membranes (Figure 3.33). A solution containing the desired crystallization condition is placed on one side of the membrane while the protein solution (5 50 l) is placed on the other. Equilibration across the membrane gradually alters the properties of the protein solution to that of the crystallization condition. The properties of the crystallization condition can be gradually changed by altering the solution

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Figure 4.1 Differentiation pathways of white and brown adipocytes. CREB: (cAMP-response-element)-binding protein; PPAR : peroxisome proliferator-activated receptor-gamma; C/EBPs: CCAAT/enhancer-binding proteins; PGC-2: peroxisome proliferator-activated receptor-gamma co-activator-2; FABP: fatty-acid binding protein; PGC-1 : peroxisome proliferator-activated receptor-gamma co-activator-1alpha; PRDM16: PR domain containing 16.

Figure 2-18: Go back in time with the History palette if you make a mistake or want to do something differently.

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By default, 3D orbit (and all three-dimensional viewpoints) displays a parallel view. In a parallel view, parallel lines never converge. The value of a parallel view is that the shapes in your drawing do not appear distorted as you zoom in. In a perspective view, parallel lines converge. You may have had an assignment in art class (long ago) to draw railroad tracks that converge to give the impression of distance. Perspective view is more realistic because railroad tracks really do seem to converge in the distance. However, when you zoom in, these objects appear distorted. Use a perspective view when you want to convey a realistic sense of depth. In general, perspective view is most useful in architectural settings. To create a perspective view in 3D orbit, right-click and choose Projection Perspective. To return to parallel view, choose Projection Parallel. For more information on perspective views, see the section Defining a Perspective View later in this chapter.

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Anytime after you begin working on a page, it s a good idea to save it. GoLive automatically records the changes you make to the Site Window, but saving the page is up to you. (Which is good, because it gives you the control.) Saving your page can be as easy as File Save, or it can take a few extra steps, depending upon how you chose to start your page.

The Express Tools offer two commands, DIMIM and DIMEX, which enable you to save (export) and retrieve (import) dimension styles. Choose Express Dimension Dimstyle Import or Dimstyle Export. For information on installing Express Tools, see Appendix A. On the CD-ROM The drawing used in the following exercise on changing dimension styles, ab15-b.dwg, is in the Drawings folder on the CD-ROM.

diagonal. A xed amount of shear may be implemented with one slightly tilted totally re ecting surface, depicted in Figure 4.25. Lenouvel and Lenouvel (1938) were probably the rst to devise the practical solid version of this interferometer. Later, Murty (1969), Saunders (1970), and several others found simplicity in this interferometer very useful in the testing of optical systems. One can vary the amount of shear using similar right-angle prisms in a cube arrangement (Murty, 1970). An index matching liquid or suitable oil inserted between the faces of the hypotenuse, permits rotation of one prism. The axis of rotation passes through both centers of curvature of split wavefronts, as illustrated in Figure 4.26. The entrance and exit faces may be made spherical so that the rays enter and exit almost without deviation. This implementation of the lateral shearing interferometer has been used to characterize cryogenic laser fusion targets (Tarvin et al., 1979).

A pop-up ad is a pop-up window used for advertising. When the program is initiated by some user action, such as a mouse click or a mouseover, a window containing an offer for some product or service appears in the foreground of the visual interface. Like all pop-ups, a pop-up ad is smaller than the background interface windows that fill the user interface are called replacement interfaces and usually resembles a small browser window with only the close, minimize, and maximize options at the top. A variation on the pop-up ad, the pop-under, is a window that loads behind the Web page that you re viewing, only to appear when you leave that Web site. (http://whatis.techtarget.com/definition/ 0,,sid9_gci891896,00.html)

When you add properties to the listing in the Block Attribute Manager, resize the dialog box so that you can see all of the columns.

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